Journal: Cell reports

Article Title: TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity

doi: 10.1016/j.celrep.2019.11.102

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant human IL-7 , BioLegend , Cat # 581908.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Software

Journal: Cell

Article Title: Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells

doi: 10.1016/j.cell.2023.08.041

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant Human IL-7 , Miltenyi Biotec , Cat#130-095-367.

Techniques: Recombinant, Modification, Activation Assay, Staining, DNA Extraction, Reverse Transcription, SYBR Green Assay, Cell Isolation, RNA Sequencing, Cell Culture, Software

Journal: BMC Cancer

Article Title: IL-6 signaling by STAT3 participates in the change from hyperplasia to neoplasia in NRP-152 and NRP-154 rat prostatic epithelial cells

doi: 10.1186/1471-2407-1-19

Figure Lengend Snippet: IL-6 expression of clones A4 (red) and D8 (green) of NRP-154 cells. Clones of NRP-154 cells were derived by limit dilution at 1 cell/well; calculated cloning efficiency was 17.8%. Clones were fixed, permeabilized, and stained with biotinylated anti-IL-6 (R&D Systems) plus streptavidin-phycoerythrin (Pharmingen), as described in Materials & Methods. Fluorescence in FL2 was analyzed on a FACScan. The IL-6 expression of the 16 clones was either high, like A4 (shown in red), or low, like D8 (shown in green). A4 cells are 8 times as fluorescent as D8 cells. The IL-6 expression of 2 of the 16 clones is shown for the sake of clarity. The fluorescence intensity of clone A4 was 10× that of clone D8 in this experiment (p < 0.001 by KS statistics). A histogram from 1 determination is shown here.

Article Snippet: Cells were blocked by incubation on ice in goat Ig (Sigma), 2 mg/ml, for 45 min. After washing twice, cells were incubated with 1 or 2 μg/10 6 cells in 100 μl biotinylated goat anti-human IL-6 receptor (ligand-affinity purified; R&D Systems) on ice for 45 min. After washing three times, cells were incubated with phycoerythrin-labeled streptavidin for 45 min on ice.

Techniques: Expressing, Clone Assay, Derivative Assay, Cloning, Staining, Fluorescence